PRV、PCV2与PPV三重PCR检测方法的建立与初步应用

为建立可同时检测猪伪狂犬病毒(PRV)、猪圆环病毒2型(PCV2)与猪细小病毒(PPV)的三重PCR方法,根据Gen Bank登录的病毒相关基因序列,选择保守区域设计引物,经过反应条件的优化,建立了可同时检测以上3种病毒的PCR诊断方法,扩增的目的片段长度分别为192 bp(PRV)、255 bp(PCV2)和759 bp(PPV)。对PRV、PCV2和PPV的核酸检测最低浓度分别为:2.5×10-2,2.2×10-3和3.7×10-2ng,证明该方法具有良好的特异性和较高的敏感性。应用该方法对56份临床样品进行检测发现,PRV、PCV2和PPV的阳性率分别为16.1%、50.0%和19.6%,二重感染率为12.5%,三重感染阳性率为3.6%。该方法的成功建立为快速高效地检测以上3种病毒提供了有效手段。     

Lipopolysaccharide induces expression of collagen VI in the rat lung

The involvement of the lung during the septic systemic inflammatory response elicited by administration of lipopolysaccharide (LPS) was investigated. Eight-week-old male Sprague–Dawley rats were injected i.p. with 15 mg/kg LPS. After 24 h, the lungs were excised to evaluate the cellular responses to LPS. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF) analysis revealed that type VI collagen (ColVI) was extremely upregulated during sepsis in the rat lung within the first 24 h of LPS administration. Upregulation of ColVI protein and its mRNA was demonstrated by Western blot analysis, real time PCR, and immunohistochemistry. To the best of our knowledge, this is the first report demonstrating the activation of ColVI in the rat lung at the early stage of systemic inflammation. Activation of ColVI might be involved in sepsis-mediated lung fibrosis at an early stage.     

基于互惠式校企合作的技能培训体系架构--以家具制造业为例

基于互惠模式,通过整合校企资源、调整组织形式等措施,对校企合作中的技能培训提出改进思路,探索家具制造业实用人才的培养模式。     

不同肥料类型对幼龄油茶生长的影响

为了给幼龄油茶的施肥提供依据,采用随机区组设计,设置果满多、上海大颂生物菌肥、希星油茶专用肥、江西绿园油茶有机专用肥、复合肥共5个施肥处理和不施肥对照,对江西奉新县幼龄油茶进行了施肥试验。调查株高、地径、冠幅、梢长、梢直径和叶绿度等指标,并分析了它们之间的关系。结果表明,施加不同类型的肥料与油茶株高、地径、冠幅、梢长、梢直径及叶绿度均呈现一定的相关性,施肥对于油茶的株高、春梢长、春梢直径及叶绿度均存在显著的影响,而油茶冠幅和地径与施肥无显著关系;在对油茶株高、地径、冠幅和春梢长度的促进作用上,以上海大颂生物菌肥为最好;在对油茶春梢直径和叶绿度的影响上,以果满多效果为最好。所以在生产上为促进油茶生长,推荐应用上海大颂生物菌肥或果满多。     

基于火强度的西山国家森林公园主要可燃物类型划分

通过外业调查和室内测定,获得西山国家森林公园主要可燃物的相关参数,输入BehavePlus模型,得到潜在火行为参数,以火强度大小为主要指标划分主要可燃物类型,结果表明:在坡度分别为25°和35°情形下,高强度火的林分为地盘松林、麻栎林和云南油杉林,中强度火的林分是华山松林和滇青冈林,低强度火的林分只有旱冬瓜林。研究结果可合理划分火险等级,实施科学的火管理。     

克拉玛依市土地荒漠化现状及其动态分析

以克拉玛依市为研究对象,采用遥感与地理信息相结合的方法获取研究区土地利用类型、荒漠化现状及2004年荒漠化状况。调查结果表明,研究区荒漠化土地在减少,在荒漠化类型及程度中,确定了研究区域以风蚀和盐渍化并存的荒漠化类型,10年间,风蚀和盐渍化荒漠化程度在减轻。     

播种时期对蒙古栎不同类型苗木出苗和生长的影响

以蒙古栎种子为材料,研究播种时期对蒙古栎不同类型苗木出苗和生长的影响,结果表明:蒙古栎在秋季不同时间播种的出苗率均在60%以上,但以早期(9月下旬)播种的出苗率最高(大于80%),早期播种的苗木在苗高、地径、侧根数、根体积和根生物量等指标上都明显要高于其它播种时期,蒙古栎在秋播裸根育苗时应适时早播;秋季播种的容器苗在所有观测的形态指标上均好于春季播种的容器苗,秋季容器育苗质量更高。     

在ERDASIMAGINE中建立邯郸市TM影像土地类型解译标志

为了准确地了解邯郸市的土地利用类型,掌握土地资源的发展动态,在ERDASIMAGINE软件支持下,将Landsat 5号卫星所得的TM4、3、2波段数据组合输出成为标准假彩色影像,对其进行几何校正、重采样、图像裁剪与镶嵌等操作,依据遥感图像特征、地物相关关系、区域特点和全国土地利用分类系统,对处理后所得的邯郸市TM影像建立土地类型解译标志。经过实地验证,其结果判正率比较高,可以满足解译精度的要求,这说明利用遥感技术建立土地利用类型解译标志是可行的。     

The Identification of QTL Associated with Cotton Fruit Branch Length Suitable for Mechanized Harvest Utilizing BSA-seq

[Objective] This study aims to identify quantitative trait loci (QTLs) associated with cotton fruit branch length by using the combination of bulked-segregant analysis (BSA) and next-generation sequencing (NGS) method. [Method] In this study, the cotton F₂ segregating population was developed with the short branch line Sujimian 125 and long-branch variety Sikang 1 as cross parents. According to the average internode length of fruit branches in middle, extreme individuals in F₂ population were divided into two groups to generate the bulked DNA samples. The whole-genome resequencing of two DNA bulks was applied to analyze the single nucleotide polymorphism (SNP) and insertion-deletion (InDel) between two groups, and the fruit branch internode length related QTLs were detected using Euclidean distance (ED). [Result] With SNP data obtained from sequencing, a 0.8 Mbp range associated region on chromosome A3 was identified; with InDel data obtained from sequencing, a 1.09 Mbp range associated region on chromosome A3 was identified. Two regions overlapped, and the overlapped range was 0.77 Mbp. [Conclusion] This result suggested that the difference between I-type fruit branch and zero-type was due to different genetic locus and pattern rather than dosage effect of the same gene.